MersV1, France, Analysis, bibRecord, 000388

In vivo footprinting of rat TAT gene: Dynamic interplay between the glucocorticoid receptor and a liver-specific factor

Identifieur interne : 000388 ( France/Analysis ); précédent : 000387; suivant : 000389

In vivo footprinting of rat TAT gene: Dynamic interplay between the glucocorticoid receptor and a liver-specific factor

Auteurs : Gildas Rigaud [France] ; Jeanne Roux [France] ; Raymond Pictet [France] ; Thierry Grange [France]

Source :

Cell [ 0092-8674 ] ; 1991.

RBID : ISTEX:AE979C40804B15853A3B95E900E7AC5799821675

English descriptors

Teeft :
- Aminotransferase, Beato, Becker, Binding site, Binding sites, Chromatin, Chromatin structure, Cleavage, Conditional binding, Contact sites, Cordingley, Dexamethasone, Dnaase, Dnaase site, Dnaase sites, Enhancer, Exclusive binding, Experimental procedures, Footprint, Footprint pattern, Footprinting, Genomic, Glucocorticoid, Glucocorticoid receptor, Glucocorticoid treatment, Grange, Grus, Hager, Hepatoma, Hepatoma cell, Hepatoma cells, Hnf5, Hnf5 binding, Hnfs, Hyperreactive, Hyperreactive bands, Lmpcr, Lmpcr products, Lower strand, Methylation, Methylation interference analysis, Mmtv, Mmtv promoter, Nuclear factors, Nucleosomal structure, Nucleosome, Nucleosomes, Nucleotide sequence, Oligonucleotide, Other proteins, Permeabilized, Permeabilized cells, Primer, Promoter, Receptor, Right hand side, Schlitz, Schmid, Steroid, Transcription, Transcription factors, Transcriptional, Tumor virus, Tyrosine aminotransferase, Upper strand, Vivo, Vivo dnaase, Vivo footprinting.

Abstract

Abstract: HNF5, a liver-specific DNA-binding protein, interacts with DNA in a manner that allows DNAase I cleavage in the middle of its recognition sequence. Using this property we have identified in vivo HNF5 bound to its sites within two glucocorticoid-responsive units of the rat tyrosine aminotransferase (TAT) gene. One HNF5-binding site is also a glucocorticoid receptor-binding site; glucocorticoid-dependent HNF5 binding could be detected at this site even though it is incompatible with glucocorticoid receptor binding. HNF5 binds within 10 min of hormone addition, indicating that it participates in transcriptional activation. In the TAT gene glucocorticoid-dependent HNF5 binding occurs where there is glucocorticoid-dependent disruption of nucleosomal structure; constitutive binding occurs in constitutively disrupted regions. These results suggest a hit-and-run mechanism of transcriptional activation by glucocorticoid receptor: the activated receptor binds its target sequence, modifies local chromatin structure, then leaves its site accessible to another factor.

Url:

https://api.istex.fr/ark:/67375/6H6-0DGM187T-R/fulltext.pdf

DOI: 10.1016/0092-8674(91)90370-E

Affiliations:

France

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 002560
to stream Istex, to step Curation: 002560
to stream Istex, to step Checkpoint: 001E73
to stream Main, to step Merge: 004A06
to stream Main, to step Curation: 004930
to stream Main, to step Exploration: 004930
to stream France, to step Extraction: 000388

Links to Exploration step

ISTEX:AE979C40804B15853A3B95E900E7AC5799821675

Le document en format XML

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<term>Chromatin</term>
<term>Chromatin structure</term>
<term>Cleavage</term>
<term>Conditional binding</term>
<term>Contact sites</term>
<term>Cordingley</term>
<term>Dexamethasone</term>
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<term>Dnaase site</term>
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<term>Experimental procedures</term>
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<term>Footprinting</term>
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<term>Glucocorticoid receptor</term>
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<term>Grus</term>
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<term>Tyrosine aminotransferase</term>
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<front><div type="abstract" xml:lang="en">Abstract: HNF5, a liver-specific DNA-binding protein, interacts with DNA in a manner that allows DNAase I cleavage in the middle of its recognition sequence. Using this property we have identified in vivo HNF5 bound to its sites within two glucocorticoid-responsive units of the rat tyrosine aminotransferase (TAT) gene. One HNF5-binding site is also a glucocorticoid receptor-binding site; glucocorticoid-dependent HNF5 binding could be detected at this site even though it is incompatible with glucocorticoid receptor binding. HNF5 binds within 10 min of hormone addition, indicating that it participates in transcriptional activation. In the TAT gene glucocorticoid-dependent HNF5 binding occurs where there is glucocorticoid-dependent disruption of nucleosomal structure; constitutive binding occurs in constitutively disrupted regions. These results suggest a hit-and-run mechanism of transcriptional activation by glucocorticoid receptor: the activated receptor binds its target sequence, modifies local chromatin structure, then leaves its site accessible to another factor.</div>
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   |wiki=    Sante
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In vivo footprinting of rat TAT gene: Dynamic interplay between the glucocorticoid receptor and a liver-specific factor

In vivo footprinting of rat TAT gene: Dynamic interplay between the glucocorticoid receptor and a liver-specific factor

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri